Publications

2024

Ohman, M.S., Albright, E.R., Gelbmann, C.B., and Kalejta, R.F. (2024). The Pentamer glycoprotein complex inhibits viral Immediate Early transcription during Human Cytomegalovirus infections. Proc. Natl. Acad. Sci. U.S.A. PMID: 39292744

Walter, R.M., Majumder, K., and Kalejta, R.F. (2024). ATRX restricts Human Cytomegalovirus (HCMV) viral DNA replication through heterochromatinization and minimizes unpackaged viral genomes. PLoS Pathog 20(9): e1012516. PMID: 39236084

Albright, E.R., and Kalejta, R.F. (2024). cGAS-STING-TBK1 signaling promotes valproic acid-responsive human cytomegalovirus immediate-early transcription during infection of incompletely differentiated myeloid cells. Viruses. PMID: 38932169

Maliano, M.R., Yetming, K.D., Kalejta, R.F. (2024). Triple lysine and nucleosome-binding motifs of the viral IE19 protein are required for human cytomegalovirus S-phase infections.mBio. PMID: 38695580.

2023

Albright E.R., Walter R.M., Saffert R.T., Kalejta R.F. (2023). NFkB and cyclic AMP response element sites mediate the valproic acid and UL138 responsiveness of the human cytomegalovirus major immediate early enhancer and promoter. J. Virol. PMID: 36856444

2022

Erickson, J., Kalejta, R.F., and Friesen, P.D. (2022) Ataxia telangiectasia-mutated is activated but not required for productive autographa californica multiple nucleopolyhedrovirus infection. J. Virol. PMID: 36314821

Iwahori S., Umaña A.C., Kalejta R.F., Murata T. (2022) Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability. J. Biol. Chem. PMID: 36150501

Albright, E.R., Morrison, K., Ranganathan, P., Carter, D.M., Nishikiori, M., Lee, J.H., Slayton, M.D., Ahlquist, P., Terhune, S.S., Kalejta, R.F. (2022). Human cytomegalovirus lytic infection inhibits replication-dependent histone synthesis and requires stem loop binding protein function. Proc. Natl. Acad. Sci. U.S.A. PMID: 35344424

2021

Albright, E.R., Mickelson, C.K. and Kalejta, R.F. (2021) Human cytomegalovirus UL138 protein inhibits the STING pathway and reduces interferon beta mRNA accumulation during lytic and latent infections. mBio. PMID: 34903048

Kalejta, R.F. (2021) Virology laboratory guidelines. J. Virol. PMID: 34319158

Palmenberg, A.C. and Kalejta, R.F. (2021) An update on gender parity trends for invited speakers at four prominent virology conference series. J. Virol. PMID: 33762415

2020

Lyon, S.M., Yetming, K.D., Paulus, C., Nevels, M., and Kalejta, R.F. (2020) Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain. mBio. PubMed PMID: 32994332.

Kalejta, R.F. and Albright, E.R. (2020) Expanding the known functional repertoire of the human cytomegalovirus pp71 protein. Front. Cell. Infect. Microbiol. PubMed PMID: 32226778.

2019

Lee, J.H., Pasquarella, J.R., Kalejta, R.F. (2019) Cell Line Models for Human Cytomegalovirus Latency Faithfully Mimic Viral Entry by Macropinocytosis and Endocytosis. J Virol. PubMed PMID: 31391271.

Gelbmann C.B., Kalejta, R.F. (2019) The Golgi sorting motifs of human cytomegalovirus UL138 are not required for latency maintenance. Virus Res. PubMed PMID: 31260705.

Lee, J.H., Kalejta, R.F. (2019) Human Cytomegalovirus Enters the Primary CD34+ Hematopoietic Progenitor Cells where it Establishes Latency by Macropinocytosis.
J Virol. PubMed PMID: 31118259.

Gelbmann C.B., Kalejta, R.F. (2019) The Membrane-Spanning Peptide and Acidic Cluster Dileucine Sorting Motif of UL138 Are Required To Downregulate MRP1 Drug Transporter Function in Human Cytomegalovirus-Infected Cells. J Virol. PMID: 30894470.

2018

Bristol, J.A., Djavadian, R., Albright, E.R., Coleman, C.B., Ohashi, M., Hayes, M., Romero-Masters, J.C., Barlow, E.A., Farrell, P.J., Rochford, R., Kalejta, R.F., Johannsen, E.C., Kenney, S.C. (2018) A cancer-associated Epstein-Barr virus BZLF1 promoter variant enhances lytic infection. PLoS Pathog. PMCID: PMC6082571.

Lyon, S.M., Kalejta, R.F. (2018) HCMV Assembly Is Totally Tubular. Dev Cell. PMID: 29634930.

Weng, C., Lee, D., Gelbmann, C.B., Van Sciver, N., Nawandar, D.M., Kenney, S.C., Kalejta, R.F. (2018) Human Cytomegalovirus Productively Replicates In Vitro in Undifferentiated Oral Epithelial Cells. J Virol. PMCID: PMC6069213.

Umaña, A.C., Iwahori, S., Kalejta, R.F. (2018) Direct Substrate Identification with an Analog Sensitive (AS) Viral Cyclin-Dependent Kinase (v-Cdk). ACS Chem Biol. PMID: 29215867.

2017

Iwahori, S., Kalejta, R.F. (2017) Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus. Virology. PMID: 28946006.

Liu, C., Clark, P.A., Kuo, J.S., Kalejta, R.F. (2017) Human Cytomegalovirus-Infected Glioblastoma Cells Display Stem Cell-Like Phenotypes. mSphere. PMID: 28656174link

Iwahori, S., Umaña, A.C., VanDeusen, H.R., Kalejta, R.F. (2017) Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem. PMID: 28289097

Kalejta R.F., Palmenberg, A.C. (2017) Gender parity trends for invited speakers at four prominent virology conference series. J Virol. PMID: 28592542

2016

Albright, E.R., Kalejta, R.F. (2016) Canonical and variant forms of histone H3 are deposited onto the human cytomegalovirus genome during lytic and latent infections. J Virol. PMID: 27605676

Lee WH, Higuchi H, Ikeda S, Macke EL, Takimoto T, Pattnaik BR, Liu C, Chu LF, Siepka SM, Krentz KJ, Rubinstein CD, Kalejta RF, Thomson JA, Mullins RF, Takahashi JS, Pinto LH, Ikeda A. (2016) Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. eLife. PMID: 27863209

Lee, S.H., Caviness, K., Albright, E.R., Lee, J.H., Gelbmann, C.B., Rak, M., Goodrum, F., Kalejta, R.F. (2016) Long and short isoforms of the human cytomegalovirus UL138 protein silence IE1 transcription and promote latency. J Virol. 90, 9483-94 PMID: 27512069

Kalejta, R.F. (2016) Sifting and Winnowing through Human Cytomegalovirus Lytic Replication and Latency. PLoS Pathog. 12(9):e1005607. PMID: 27657905

Kuny, C.V., Kalejta, R.F. (2016) Human cytomegalovirus can procure deoxyribonucleotides for viral DNA replication in the absence of retinoblastoma protein phosphorylation. J. Virol. 90, 8634-43 PMID: 27440891

2015

Lee, S.H., Albright, E.R., Lee, J-H., Jacobs, D., and Kalejta, R.F. (2015) Cellular defense against latent colonization foiled by human cytomegalovirus UL138 protein. Sci. Adv. 1(10):e1501164 PMCID: PMC4681346

VanDeusen, H.R., and Kalejta, R.F. (2015) Deficiencies in cellular processes modulated by the retinoblastoma protein do not account for reduced human cytomegalovirus replication in its absence. J. Virol. 89, 11965-74 PMCID: PMC4645314

Iwahori, S., Hakki, M., Chou, S., and Kalejta, R.F.  (2015)  Molecular determinants for the inactivation of the retinoblastoma tumor suppressor by the viral cyclin-dependent kinase UL97.  J. Biol. Chem. 290, 19666-19680  PMCID:  PMC4528131

VanDeusen, H.R., and Kalejta, R.F.  (2015)  The retinoblastoma tumor suppressor promotes efficient human cytomegalovirus lytic replication.  J. Virol. 89, 5012-5021  PMCID:  PMC4403481

2014

Winkler, L.L., and Kalejta, R.F.  (2014)  The 19S proteasome activator promotes human cytomegalovirus immediate early gene expression through proteolytic and non-proteolytic mechanisms.  J. Virol. 88, 11782-11790  PMCID: PMC4178716

Qin, Q., Lee, S.H., Liang, R., and Kalejta, R.F.  (2014)  Insertion of myeloid-active elements into the human cytomegalovirus major immediate early promoter is not sufficient to drive its activation upon infection of undifferentiated myeloid cells.  Virology 448C, 125-132.  PMCID: PMC3857587

2013

Sun, X., Bristol, J.A., Iwahori, S., Hagemeier, S.R., Meng, Q., Barlow, E.A., Fingeroth, J.D., Tarakanova,V.L., Kalejta, R.F., and Kenney, S.C.  (2013)  Hsp90 inhibitor 17-DMAG decreases expression of conserved herpesvirus protein kinases and reduces virus production in Epstein-Barr virus-infected cells.  J. Virol. 87, 10126-10138.  PMCID: PMC3754017

Qin, Q., Penkert, R.R., and Kalejta, R.F.  (2013)  Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.  J. Virol. 87, 9886-9894.  PMCID: PMC3754107

Albright, E.R., and Kalejta, R.F.  (2013)  Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.  J. Virol. 87, 9802-9812.  PMCID: PMC3754112

Penkert, R.R., and Kalejta, R.F.  (2013)  Human embryonic stem cell lines model experimental human cytomegalovirus latency. mBio 4(3):e00298-13.  PMCID: PMC3663570

Winkler, L.L., Hwang, J., and Kalejta, R.F.  (2013)  Ubiquitin-independent proteasomal degradation of tumor suppressors by human cytomegalovirus pp71 requires the 19S regulatory particle.  J. Virol. 87, 4665-4671.  PMCID: PMC3624388

Kalejta, R.F.  (2013)  Pre-Immediate Early Tegument Protein Functions.  In Cytomegaloviruses: From Molecular Pathogenesis to Intervention, Volume 1, Chapter 9; M.J. Reddehase, ed. (Norfolk, UK, Caister Academic Press), pp.141-151;  PMCID: none link

2012

Penkert, R.R., and Kalejta, R.F.  (2012)  Tale of a tegument transactivator: the past, present, and future of HCMV pp71. Future Virology, 7, 855-869;  PMCID: PMC3558950

Dziurzynski, K., Cjang, S.M., Heimberger, A.B., Kalejta, R.F., McGregor Dallas, S.R., Smit, M., Soroceanu, L., Cobbs, C.S., and the HCMV and Gliomas Symposium.  (2012) Consensus on the role of human cytomegalovirus in glioblastoma.  Neuro Oncol. 14, 246-255.  PMCID: PMC3280809

Lee, S.H., Kalejta, R.F., Kerry, J., Semmes, O.J., O’Connor, C.M., Khan, Z., Garcia, B.A., Shenk, T., and Murphy, E.  (2012)  BclAF1 restriction factor is neutralized by proteasomal degradation and microRNA repression during human cytomegalovirus infection.  Proc. Natl. Acad. Sci. USA, 109, 9575-9580  PMCID: PMC3386064

Ranganathan, P., Clark, P.A., Kuo, J.S., Salamat, M.S., and Kalejta, R.F.  2012.  Significant association of multiple human cytomegalovirus genomic loci with glioblastoma multiforme samples.  J. Virol. 86, 854-864 PMCID: PMC3255835.

2011

Hwang, J., and Kalejta, R.F.  2011.  In vivo analysis of protein sumoylation induced by a viral protein: Detection of HCMV pp71-induced Daxx sumoylation  Methods 55, 160-165  PMCID: PMC3208771

Hwang, J., Winkler, L., and Kalejta, R.F.  (2011)  Ubiquitin-independent proteasomal degradation during oncogenic viral infections.  Biochim. Biophys. Acta 1816 (2) 147-157  PMCID:  PMC3193896

Penkert, R.R., and Kalejta, R. F. (2011) Tegument protein control of latent herpesvirus establishment and animation. Herpesviridae 2:3 PMCID: PMC3063196

Hwang, J., Saffert, R. T., and Kalejta, R. F. (2011) Elongin B-mediated epigenetic alteration of viral chromatin correlates with efficient human cytomegalovirus gene expression and replication. mBio 2(2):e00023-11.doi:10.1128/mBio.00023-11  PMCID: PMC3063379

2010

Kuny, C.V., Chinchilla, K., Culbertson, M.R., and Kalejta, R.F. (2010)  Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases.  PLoS Pathog. 6(9): e1001092. doi10.1371/journal.ppat.1001092.  PMCID: PMC2936540

Penkert, R.R., and Kalejta, R.F.  (2010)  Nuclear localization of tegument-delivered pp71 in human cytomegalovirus infected cells is facilitated by one or more factor present in terminally differentiated fibroblasts.  J. Virol. 84 (19) 9853-63.  PMCID: PMC2937784

Saffert, R.T., Penkert, R.R., and Kalejta, R.F.  (2010)  Cellular and viral control over the initial events of human cytomegalovirus experimental latency in CD34+ cell.  J. Virol. May 5 2010; 84: (11) 5594-5604. Epub 2010 Mar 24 PMCID: PMC2876595

Meng Q., Hagemeier S.R., Kuny C.V., Kalejta R.F., and Kenney S.C.  Simian virus 40 T/t antigens and lamin A/C small interfering RNA rescue the phenotype of an Epstein-Barr virus protein kinase (BGLF4) mutant.  J. Virol. May 2010; 84: 4524-4533. Epub 2010 Feb 10 PMCID: PMC2863785

2009

Hume A.J., Kalejta R.F.  Regulation of the retinoblastoma proteins by the human herpesviruses. Cell Div. 2009  Jan 15;4:1.PMCID: PMC 2636798

Hwang J, Kalejta R.F., Human cytomegalovirus protein pp71 induces Daxx SUMOylation. J Virol 2009 Jul;83(13):6591-8. Epub 2009 Apr 15.PMCID: PMC 2698545

Kamil J.P., Hume A.J., Jurak I., Münger K., Kalejta R.F., Coen D.M. Human papillomavirus 16 E7 inactivator of retinoblastoma family proteins complements human cytomegalovirus lacking UL97 protein kinase. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16823-8. Epub 2009 Sep 15. PMCID: PMC2757844

2008

Hume A.J., Finkel J.S., Kamil J.P., Coen D.M., Culbertson M.R., Kalejta R.F. Phosphorylation of retinoblastoma protein by viral protein with cyclin-dependent kinase function. Science. 2008 May 9;320(5877):797-9. PMID: 18467589

Kalejta R.F. Tegument proteins of human cytomegalovirus. Microbiol Mol Biol Rev. 2008 Jun;72(2):249-65, table of contents. Review. PMCID: PMC2415745

Kalejta R.F. Functions of human cytomegalovirus tegument proteins prior to immediate early gene expression. Curr Top Microbiol Immunol. 2008;325:101-15. Review. PMID: 18637502

Saffert R.T., Kalejta R.F. Promyelocytic leukemia-nuclear body proteins: herpesvirus enemies, accomplices, or both? Future Virol. 2008 May 1;3(3):265-277. PMCID: PMC2744987

2007

Hwang J., Kalejta R.F. Proteasome-dependent, ubiquitin-independent degradation of Daxx by the viral pp71 protein in human cytomegalovirus-infected cells. Virology. 2007 Oct 25;367(2):334-8. Epub 2007 Jun 27. PMID: 17590404

Saffert R.T., Kalejta R.F. Human cytomegalovirus gene expression is silenced by Daxx-mediated intrinsic immune defense in model latent infections established in vitro. J Virol. 2007 Sep;81(17):9109-20. Epub 2007 Jun 27.PMCID: PMC1951389

2006

Saffert R.T., Kalejta R.F. Inactivating a cellular intrinsic immune defense mediated by Daxx is the mechanism through which the human cytomegalovirus pp71 protein stimulates viral immediate-early gene expression. J Virol. 2006 Apr;80(8):3863-71. PMCID: PMC 1440479

2004

Kalejta R.F. Human cytomegalovirus pp71: a new viral tool to probe the mechanisms of cell cycle progression and oncogenesis controlled by the retinoblastoma family of tumor suppressors. J Cell Biochem. 2004 Sep 1;93(1):37-45. Review. PMID: 15352160

Postdoc

Kalejta R.F., Shenk T. The human cytomegalovirus UL82 gene product (pp71) accelerates progression through the G1 phase of the cell cycle.J Virol. 2003 Mar;77(6):3451-9. PMCID: PMC149542

Kalejta R.F., Shenk T. Proteasome-dependent, ubiquitin-independent degradation of the Rb family of tumor suppressors by the human cytomegalovirus pp71 protein. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3263-8. Epub 2003 Mar 7. PMCID: PMC152280

Kalejta R.F., Bechtel J.T., Shenk T. Human cytomegalovirus pp71 stimulates cell cycle progression by inducing the proteasome-dependent degradation of the retinoblastoma family of tumor suppressors. Mol Cell Biol. 2003 Mar;23(6):1885-95. PMCID: PMC149485

Kalejta R.F., Shenk T. Manipulation of the cell cycle by human cytomegalovirus. Front Biosci. 2002 Jan 1;7:d295-306. Review. PMID: 11779699

Tao W., Pennica D., Xu L., Kalejta R.F., Levine A. J. Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. Genes Dev. 2001 Jul 15;15(14):1796-807. PMCID: PMC312736

Beavis A.J., Kalejta R.F. Simultaneous analysis of the cyan, yellow and green fluorescent proteins.Current Protocols in Cytometry. 2001 1.16.1-1.16.12 link

Beavis A.J., Kalejta R.F. Simultaneous analysis of the cyan, yellow and green fluorescent proteins by flow cytometry using single-laser excitation at 458 nm. Cytometry. 1999 Sep 1;37(1):68-73. PMID: 10451508

Kalejta R.F., Brideau A.D., Banfield B.W., Beavis A.J. An integral membrane green fluorescent protein marker, Us9-GFP, is quantitatively retained in cells during propidium iodide-based cell cycle analysis by flow cytometry. Exp Cell Res. 1999 Apr 10;248(1):322-8. PMID: 10094838

Kalejta R.F., Shenk T., Beavis A.J. Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cells and cell cycle analysis by flow cytometry. Cytometry. 1997 Dec 1;29(4):286-91. PMID: 9415410

Graduate

Kalejta R.F., Li X., Mesner L.D., Dijkwel P.A., Lin H.B., Hamlin J.L. Distal sequences, but not ori-beta/OBR-1, are essential for initiation of DNA replication in the Chinese hamster DHFR origin. Mol Cell. 1998 Dec;2(6):797-806. PMID: 9885567

Kalejta R.F., Hamlin J.L. The dual effect of mimosine on DNA replication. Exp Cell Res. 1997 Feb 25;231(1):173-83. PMID: 9056424

Kalejta R.F., Hamlin J.L. Composite patterns in neutral/neutral two-dimensional gels demonstrate inefficient replication origin usage. Mol Cell Biol. 1996 Sep;16(9):4915-22. PMCID: PMC231493

Kalejta R.F., Lin H.B., Dijkwel P.A., Hamlin J.L. Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques. Mol Cell Biol. 1996 Sep;16(9):4923-31. PMCID: PMC231494